- Aseptic Precautions = this prevents pathogens and unwanted bacteria from contaminating the substrates and products
- Optimum temperature = enzymes produced by the microorganism function best at this temperature, so to produce more yield, this temperature is controlled
- pH Levels = enzymes also function best at a certain pH, so this is similarly controlled by the fermenter
- Oxygenation = Oxygen is needed in the reaction since it is aerobic
- Agitation = this ensures that the microorganisms are distributed evenly throughout the fermenter, and prevents clusters of them from forming; also ensures even temperature and nutrient concentration
credits to Nik
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