5.14 Humulin

The bacterial cell containing the recombinant DNA with the human genes (in this case the production of insulin) can be injected into a fermenter.

In this fermentor chamber we need to consider:

• -Nutrient  - (Used to manufacture insulin protein) 
•  Temperature - (Optimal temperature for bacteria growth ->Increase in population where the bacteria will then switch on the gene for insulin and manufacture protein insulin. )
•  pH
•  Gases 

It will then be necessary to remove the product and carry out purification ( downstream processing)

Genetically engineered human insulin is called Humulin.